Variations in cell density significantly impact cell behavior, influencing growth, membrane properties, gene expression, and nanoparticle (NP) interactions. Despite their biological relevance, these variations are often overlooked in nanomedicine and toxicity studies. This research investigates how cell density affects NP uptake using bioprinted A549 epithelial cell cultures. By analyzing NP internalization through confocal microscopy and 3D imaging, we aim to understand how density-driven changes in cell properties modulate NP interactions. These findings will contribute to improving in vitro models, optimizing drug delivery strategies, and refining nanotoxicity assessments for safer biomedical applications. 

Funding source: 

National Center of Competence in Research (NCCR) Bio-inspired Materials: NCCR | Adolphe Merkle Institute | Université de Fribourg