Interaction of Engineered Nanoparticles with proteins

If nanoparticles are to be used in medical applications, especially if they are administered systemically, the requirements are particularly severe. The particle hydrodynamic size must be as small as possible and the particle surface has to be modified in order to ensure colloidal stability in the blood compartment and long plasma half-lives by minimizing or delaying the opsonization processes. In recent years, it has become clear that our understanding of the interaction of nanoscale objects with living matter, even at the level of single cells, has not kept pace with the explosive development of nanoscience in the past. In general, material surfaces are modified by the adsorption of biomolecules such as proteins in a biological environment, and it is hypothesized that cellular responses to materials in a biological medium reflect the adsorbed biomolecule layer, rather than the material itself.

It is our goal to obtain a better understanding of the biological effects of nanoparticles. This requires not only the identification of adsorbed molecules but a better knowledge of binding properties of proteins (and other molecules) that associate with the particles as a function of the particle properties such as size and/or surface. 


Principal investigator

Involved people

External partners

Dr. Marc Moniatte

Proteomics Core Facility, EPFL

Publications

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